The Self-Inactivating KamiCas9 System for the Editing of CNS Disease Genes.
Merienne N, Vachey G, de Longprez L, Meunier C, Zimmer V, Perriard G, Canales M, Mathias A, Herrgott L, Beltraminelli T, Maulet A, Dequesne T, Pythoud C, Rey M, Pellerin L, Brouillet E, Perrier AL, du Pasquier R, Deglon N
Cell Rep. 2017 Sep 19;20(12):2980-2991. doi: 10.1016/j.celrep.2017.08.075.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87874 | SIN-CMV-Ca9-WPRE | Transfer plasmid for the production of lentiviral vectors |
| 87876 | SIN-PGK-Cas9-V5-WPRE | To produce lentiviral vector for Cas9 editing |
| 87885 | SIN-cPPT-H1-sgHTT1-PGK-mCherry-WPRE | To produce lentiviral vector for HTT editing |
| 87886 | SIN-PGK-Cas9-WPRE | To produce Lentiviral vector for gene editing |
| 87904 | SIN-CMV-Cas9-V5-WPRE | To produce lentiviral vector for gene editing |
| 87905 | pENTR4-Cas9-V5 | Cloning vector |
| 87906 | pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3 | sgRNA targeting GFP |
| 87908 | pENTR221-H1-sgGFP1-7SK-sgCas9 | multiple sgRNA |
| 87912 | SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE | Kamicas9 lentiviral vector |
| 87917 | pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9 | Gateway cloning |
| 87919 | SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE | HIV-1 SIN lentiviral transfer vector |