Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase.
Eggenschwiler R, Moslem M, Fraguas MS, Galla M, Papp O, Naujock M, Fonfara I, Gensch I, Wahner A, Beh-Pajooh A, Mussolino C, Tauscher M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, Cathomen T, Cantz T
Sci Rep. 2016 Dec 2;6:38198. doi: 10.1038/srep38198.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86003 | AAT-PB-CG2APtk | homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette |
| 86004 | AAT-PB-CD2APtk | homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette |
| 86005 | AAT_g1 gRNA | plasmid vector encoding for U6-driven AAT_g1 gRNA |
| 86006 | AAT_g2 gRNA | plasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific) |
| 86007 | Lenti CGIP + Z-AAT target | lenti reporter plasmid with Z-AAT-specific gRNA target-sequence, to be used with tGFP #26864 |
| 86008 | Lenti CGIP + M-AAT target | lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864 |
| 86009 | Lenti CGIP + TTR target | lenti reporter plasmid with TTR_V30M-specific gRNA target-sequence, to be used with tGFP #26864 |