CDK-regulated dimerization of M18BP1 on a Mis18 hexamer is necessary for CENP-A loading.
Pan D, Klare K, Petrovic A, Take A, Walstein K, Singh P, Rondelet A, Bird AW, Musacchio A
Elife. 2017 Jan 6;6. pii: e23352. doi: 10.7554/eLife.23352.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87803 | pcDNA5-EGFP-NLS-P2A-mCherry-PTS1 | Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest. |
| 87827 | pcDNA5-EGFP-NLS-T2A-mCherry-PTS1 | Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest. |
| 87828 | pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1 | High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest. |
| 87829 | pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1 | High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest. |