Synthetic Chemical Inducers and Genetic Decoupling Enable Orthogonal Control of the rhaBAD Promoter.
Kelly CL, Liu Z, Yoshihara A, Jenkinson SF, Wormald MR, Otero J, Estevez A, Kato A, Marqvorsen MH, Fleet GW, Estevez RJ, Izumori K, Heap JT
ACS Synth Biol. 2016 Oct 21;5(10):1136-1145. Epub 2016 Jun 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87766 | pCK300 | empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI |
| 87767 | pCK301 | E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI |
| 87768 | pCK302 | As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used |