Engineered DNA ligases with improved activities in vitro.
Wilson RH, Morton SK, Deiderick H, Gerth ML, Paul HA, Gerber I, Patel A, Ellington AD, Hunicke-Smith SP, Patrick WM
Protein Eng Des Sel. 2013 Jul;26(7):471-8. doi: 10.1093/protein/gzt024. Epub 2013 Jun 10.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87741 | pCA24N-ligase | IPTG-inducible expression of T4 DNA ligase for protein purification |
| 87742 | pCA24N-p50-ligase | IPTG-inducible expression of p50-ligase fusion for protein purification |
| 87743 | pCA24N-ligase-cTF | IPTG-inducible expression of ligase-cTF fusion for protein purification |
| 87744 | pCA24N-ligase-pprA | IPTG-inducible expression of ligase-pprA fusion for protein purification |
| 87745 | pCA24N-sso7d-ligase | IPTG-inducible expression of sso7d-ligase fusion for protein purification |
| 87746 | pCA24N-ligase-NLS-cTF | IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification |