One-step generation of conditional and reversible gene knockouts.
Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK
Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84538 | pUC118-FLIP-Puro | a mock vector for generating FLIP targeting vectors |
| 84539 | pUC118-FLIP-FlpE-Puro | a mock vector for generating FLIP-FlpE targeting vectors |
| 84540 | pUC118-mCtnnb1-FLIP-Puro | CRISPR - FLIP targeting vector for mouse beta-catenin |
| 84541 | pUC118-mCtnnb1-FLIP-FlpE-Puro | CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin |
| 84542 | gRNA-mCtnnb1 | mCtnnb1 gRNA expressing vector |