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RefSOFI for Mapping Nanoscale Organization of Protein-Protein Interactions in Living Cells.

Hertel F, Mo GC, Duwe S, Dedecker P, Zhang J
Cell Rep. 2016 Jan 12;14(2):390-400. doi: 10.1016/j.celrep.2015.12.036. Epub 2015 Dec 31. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
87609pcDNA3-Lyn-FRB-DMVF-181-NFRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.
87610pcDNA3-LifeAct-FRB-DMVF-181-NFRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.
87611pcDNA3-FKBP-DMVF-181-CFKBP-tagged C-terminal Dronpa fragment for BiFC.
87612pcDNA3-Venus-CAAXPlasma membrane-targeted Venus (YFP).
87613pcDNA3-LifeAct-VenusActin-targeted Venus (YFP).
87614pcDNA3-H2B-VenusVenus (YFP) fused to histone H2B.
87615pcDNA3-Lyn-FRB-Venus-173-NFRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to plasma membrane.
87616pcDNA3-LifeAct-FRB-Venus-173-NFRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.
87617pcDNA3-FKBP-Venus-173-CFKBP-tagged C-terminal Venus (YFP) fragment for BiFC.
87618pcDNA3-Venus-173-N-ORAI1N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.
87619pcDNA3.1-STIM1-Venus-173-CFull-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.
87620pcDNA3-ER-rsTagRFPEndoplasmic reticulum (ER)-targeted rsTagRFP.
87621pcDNA3-HER3-DMVF-181-RC-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.
87622pcDNA3-HER2-DMVF-181-NN-terminal Dronpa fragment fused to the C-terminus of full-length HER2; for BiFC.

Antibodies from Article