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Identification and characterization of the methyl arginines in the fragile X mental retardation protein Fmrp.

Stetler A, Winograd C, Sayegh J, Cheever A, Patton E, Zhang X, Clarke S, Ceman S
Hum Mol Genet. 2006 Jan 1;15(1):87-96. Epub 2005 Nov 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
87913p-EGFP-C1-Flag-mFmr1(A)Expresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.
87914p-EGFP-C1-Flag-mFmr1(D)Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.
87929p-EGFP-C1-Flag-mFmr1(wt)Expresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.

Antibodies from Article