Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84739 | pY108 (lenti-AsCpf1) | Lenti virus delivery of AsCpf1 and crRNA guide |
| 84740 | pY109 (lenti-LbCpf1) | Lenti virus delivery of LbCpf1 and crRNA guide |
| 84741 | pY026 | Expresses huAsCpf1 and crRNA guide |
| 84742 | pY027 | Expresses huLbCpf1 and crRNA guide |
| 84743 | pY094 | Expresses huAsCpf1-T2A-GFP and crRNA guide |
| 84744 | pY095 | Expresses huLbCpf1-T2A-GFP and crRNA guide |
| 84745 | pY30 | Expresses huAsCpf1-P2A-puro and crRNA guide |
| 84746 | pY31 | Expresses huLbCpf1-P2A-puro and crRNA guide |
| 90095 | 6His-MBP-TEV-huAsCpf1 | Bacterial expression plasmid for protein purification |
| 90096 | 6His-MBP-TEV-huLbCpf1 | Bacterial expression plasmid for protein purification |