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A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors.

Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, Hacohen N, Walker BD
Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
83929pLenti-sgALCAMLentiviral vector expressing an sgRNA targeting ALCAM. NOTE: This plasmid does NOT contain Cas9.
83930pLenti-sgCCR5Lentiviral vector expressing an sgRNA targeting CCR5 NOTE: This plasmid does NOT contain Cas9.
83932pLenti-sgSLC35B2Lentiviral vector expressing an sgRNA targeting SLC35B2 NOTE: This plasmid does NOT contain Cas9.
83933pLenti-sgTPST2Lentiviral vector expressing an sgRNA targeting TPST2 NOTE: This plasmid does NOT contain Cas9.
83934pLenti-sgCTRL-1Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.
83935pLenti-sgCTRL-2Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.
83936pLenti-sgCTRL-3Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.
83939ALCAM-p2A-E2CrimsonLentiviral vector expressing ALCAM-p2A-E2Crimson
83940RAP2A-p2A-E2CrimsonLentiviral vector expressing RAP2A-p2A-E2Crimson
83941SLC35B2-p2A-E2CrimsonLentiviral vector expressing SLC35B2-p2A-E2Crimson
83944pLenti-sgRELALentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

Antibodies from Article