Naturally Occurring Off-Switches for CRISPR-Cas9.
Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR
Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85677 | pEJS427-pCSDest2-AcrE2 | Mammalian expression of anti-CRISPR protein from Type I-E |
| 85678 | pEJS430-pCSDest2-AcrIIC1Boe | Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Boe from Brackiella oedipodis |
| 85679 | pEJS433-pCSDest2-AcrIIC1Nme | Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidis |
| 85712 | pEJS436-pCSDest2-AcrIIC2Nme | Mammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidis |
| 85713 | pEJS443-pCSDest2-AcrIIC3Nme | Mammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidis |
| 85714 | pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere | Telomere-targeting Nme sgRNA under U6 promoter |
| 85715 | pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere | Telomere-targeting Spy sgRNA under U6 promoter |
| 85716 | pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-one | Expresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA |
| 85717 | pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one | Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA |
| 85748 | pEJS507-pCDest2-noAcr-mTagBFP2-IRES | Expresses mTagBFP2 only; used as a control |
| 85749 | pEJS481-pCDest2-AcrE2-mTagBFP2-IRES | Expresses anti-CRISPR protein from Type I-E and mTagBFP2 |
| 85750 | pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES | Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2 |
| 87448 | pCSDest2-NmeCas9-NLS-3XHA-NLS | Mammalian expression of wild type Nme Cas9 |