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A bright and photostable photoconvertible fluorescent protein.

McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
Nat Methods. 2009 Feb . 6(2):131-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
20341pRSETa mEos2bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)
54510mEos2-C1Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
54632mEos2-Display-5Localization: Cytoplasm, Excitation: 505 / 569, Emission: 516 / 581
54662mEos2-N1Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
54750mEos2-Peroxisomes-2Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581
54809mEos2-Lifeact-7Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581
57340mEos2-Actin-C-18Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581
57344mEos2-Alpha-5-Integrin-12Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
57350mEos2-Beta-Catenin-20Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581
57351mEos2-CAF1-C-10Localization: Chromatin Assembly Factor 1, Excitation: 505 / 569, Emission: 516 / 581
57352mEos2-Calnexin-N-14Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581
57353mEos2-Calreticulin-N-10Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581
57354mEos2-CathespinB-6Localization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581
57355mEos2-Caveolin-C-10Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581
57356mEos2-CD9-10Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581
57357mEos2-CD36-C-10Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581
57358mEos2-CD151-7Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581
57359mEos2-CENPALocalization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581
57360mEos2-CENPB-N-22Localization: Nucleus/Centromeres, Excitation: 505 / 569, Emission: 516 / 581
57361mEos2-Clathrin-15Localization: Clathrin Vesicles, Excitation: 505 / 569, Emission: 516 / 581
57362mEos2-Coronin1B-C-10Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 581
57363mEos2-C-Src-7Localization: Membrane/Vesicles, Excitation: 505 / 569, Emission: 516 / 581
57364mEos2-Cx26-7Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581
57365mEos2-Cx43-7Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581
57367mEos2-Dectin1A-C-10Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581
57368mEos2-Dectin1A-N-10Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581
57370mEos2-Desmocollin1-N-18Localization: Cadherin, Excitation: 505 / 569, Emission: 516 / 581
57371mEos2-EB1-C-18Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581
57372mEos2-EB3-7Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581
57373mEos2-ER-5Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581
57376mEos2-Fascin-C-10Localization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581
57377mEos2-Fibrillarin-7Localization: Nucleoli, Excitation: 505 / 569, Emission: 516 / 581
57378mEos2-FilaminA-C-14Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
57379mEos2-FilaminA-N-9Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
57382mEos2-Golgi-7Localization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581
57383mEos2-H1-10Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581
57384mEos2-H2B-6Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581
57386mEos2-Hec1-C-12Localization: Kinetochore, Excitation: 505 / 569, Emission: 516 / 581
57389mEos2-Integrin-Alpha2-N-18Localization: Integrin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
57390mEos2-Integrin-Beta1-N-18Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
57391mEos2-Integrin-Beta3-N-18Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
57392mEos2-Keratin-17Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581
57395mEos2-LaminB1-10Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581
57396mEos2-LC-Myosin-N-7Localization: Light Chain Myosin, Excitation: 505 / 569, Emission: 516 / 581
57397mEos2-Lysosomes-20Localization: Lysosome Membrane, Excitation: 505 / 569, Emission: 516 / 581
57398mEos2-MannII-N-10Localization: Mannosidase/Golgi, Excitation: 505 / 569, Emission: 516 / 581
57399mEos2-MAP4-C-10Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581
57401mEos2-Mito-7Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581
57409mEos2-Paxillin-22Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
57410mEos2-PMP-N-10Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581
57432mEos2-Tubulin-C-18Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581
57434mEos2-VASP-N-10Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
57435mEos2-Vimentin-7Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581
57438mEos2-Vinculin-14Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581
57701Dendra2-Actin-C-18Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573
57715Dendra2-EB3-7Localization: MT End Binding Protein, Excitation: 490 / 553, Emission: 507 / 573
57721Dendra2-Gamma-Tubulin-17Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 573
57722Dendra2-Golgi-7Localization: Golgi, Excitation: 490 / 553, Emission: 507 / 573
57726Dendra2-Keratin-17Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 490 / 553, Emission: 507 / 573
57728Dendra2-LaminB1-10Localization: Nuclear Envelope, Excitation: 490 / 553, Emission: 507 / 573
57744Dendra2-VASP-5Localization: Actin/Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573
57745Dendra2-Vimentin-7Localization: Intermediate Filaments, Excitation: 490 / 553, Emission: 507 / 573
57748Dendra2-Vinculin-23Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573
57750Dendra2-Zyxin-6Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Antibodies from Article