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Rapid and inexpensive evaluation of nonstandard amino acid incorporation in Escherichia coli.

Monk JW, Leonard SP, Brown CW, Hammerling MJ, Mortensen C, Gutierrez AE, Shin NY, Watkins E, Mishler DM, Barrick JE
ACS Synth Biol. 2016 Sep 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
113642pRYGPositive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.
113643pRXGThis construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.
113644pRF0G-TyrPlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.
113645pRF0G-IodoYPlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

Antibodies from Article