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Vegfc acts through ERK to induce sprouting and differentiation of trunk lymphatic progenitors.

Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
67691pToneDestattR4-attR3 Destination cassette in Tol1 backbone
67692pToneTPFor in vitro transcription of mRNA encoding Tol1 Transposase
67693pTol1-UAS-nlstdTomatoTol1 expression plasmid with UAS-driven myc tagged nlsTdTomato
67695pME-Fv2E-flt4-nsmiddle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domain
67696pME-Fv2E-flt4um200-nsmiddle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain
67697pCS-Fv2Eflt4-2a-tdTomatoExpression construct
67698pCS-Fv2Eflt4um200-2a-tdTomatoExpression construct
67699p3E-2a-6MTnlstdTomato3' entry clone with 6xmyc nuclear localized tdTomato
67704pCS-flt4um159-HAExpression construct
67706pTol1-uas-mAKT-2a-nlstdTomatoTol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomato
67707p3E-2a-tdTomato3' entry 2A-tdTomato
67708p3EHA3' entry HA tag
67710pME-6MTzactMap2k2bMiddle entry clone
104721pTol1:uas-myc-act-map2k2b-2a-tdTomatoUAS-driven expression of activated MEK

Antibodies from Article