Recombinase-Mediated Cassette Exchange (RMCE)-in Reporter Cell Lines as an Alternative to the Flp-in System.
Callesen MM, Berthelsen MF, Lund S, Fuchtbauer AC, Fuchtbauer EM, Jakobsen JE
PLoS One. 2016 Aug 19;11(8):e0161471. doi: 10.1371/journal.pone.0161471. eCollection 2016.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 67275 | Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR | For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines |
| 67276 | Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3 | RMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PA |