SRGAP2 and Its Human-Specific Paralog Co-Regulate the Development of Excitatory and Inhibitory Synapses.
Fossati M, Pizzarelli R, Schmidt ER, Kupferman JV, Stroebel D, Polleux F, Charrier C
Neuron. 2016 Jul 20;91(2):356-69. doi: 10.1016/j.neuron.2016.06.013. Epub 2016 Jun 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222613 | pCAG SRGAP2A_EVH1(P339L F342C) | Expresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1 |
| 222614 | pCAG_SRGAP2A_SH3(W765A)_HA | Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin. |
| 222616 | pCAG_SRGAP2A_SH3(W765A)_GFP | Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin. |
| 222617 | pCAG_SRGAP2A_GAP(R527L_K566A) | Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP. |