Cas9-assisted recombineering in C. elegans: genome editing using in vivo assembly of linear DNAs.
Paix A, Schmidt H, Seydoux G
Nucleic Acids Res. 2016 Sep 6;44(15):e128. doi: 10.1093/nar/gkw502. Epub 2016 Jun 1.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
87240 | AP553‐1 | gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence) |
87247 | AP1297‐1 | gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence) |
87248 | AP1298‐1 | gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence) |