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SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast.

Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA
Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
87219pRS416 Hsh155 WTPlasmid used to maintain viability after deletion of genomic copy of HSH155.
87220pRS414 Hsh155 WTEncodes Hsh155 WT for expression in yeast
87266pRS414 Hsh155 E291DEncodes Hsh155 E291D for expression in yeast
87267pRS414 Hsh155 Y292CEncodes Hsh155 Y292C for expression in yeast
87268pRS414 Hsh155 R294CEncodes Hsh155 R294C for expression in yeast
87269pRS414 Hsh155 R294LEncodes Hsh155 R294L for expression in yeast
87270pRS414 Hsh155 N295DEncodes Hsh155 N295D for expression in yeast
87271pRS414 Hsh155 H331DEncodes Hsh155 H331D for expression in yeast
87272pRS414 Hsh155 H331QEncodes Hsh155 H331Q for expression in yeast
87273pRS414 Hsh155 K335EEncodes Hsh155 K335E for expression in yeast
87274pRS414 Hsh155 K335NEncodes Hsh155 K335N for expression in yeast
87275pRS414 Hsh155 K335REncodes Hsh155 K335R for expression in yeast
87276pRS414 Hsh155 K335TEncodes Hsh155 K335T for expression in yeast
87277pRS414 Hsh155 P369EEncodes Hsh155 P369E for expression in yeast
87278pRS414 Hsh155 P369KEncodes Hsh155 P369K for expression in yeast
87279pRS414 Hsh155 G409EEncodes Hsh155 G409E for expression in yeast
87280pRS414 Hsh155 K410NEncodes Hsh155 K410N for expression in yeast
87281pRS414 Hsh155 D450GEncodes Hsh155 D450G for expression in yeast
87282pRS414 Hsh155 R294L/H331DEncodes Hsh155 R294L H331D for expression in yeast
87283pRS414 Hsh155 R294L/K335EEncodes Hsh155 R294L K335E for expression in yeast
87284pRS414 Hsh155 R294L/D450GEncodes Hsh155 R294L D450G for expression in yeast
87285pRS414 Hsh155 N295D/H331DEncodes Hsh155 N295D H331D for expression in yeast
87286pRS414 Hsh155 N295D/K335EEncodes Hsh155 N295D K335E for expression in yeast
87287pRS414 Hsh155 N295D/D450GEncodes Hsh155 N295D D450G for expression in yeast
87288pGADT7 Hsh155 WTEncodes Y2H Gal4 activation domain for Hsh155 WT
87290pGADT7 Hsh155 E291DEncodes Y2H Gal4 activation domain for Hsh155 E291D
87291pGADT7 Hsh155 Y292CEncodes Y2H Gal4 activation domain for Hsh155 Y292C
87292pGADT7 Hsh155 R294CEncodes Y2H Gal4 activation domain for Hsh155 R294C
87293pGADT7 Hsh155 R294LEncodes Y2H Gal4 activation domain for Hsh155 R294L
87294pGADT7 Hsh155 N295DEncodes Y2H Gal4 activation domain for Hsh155 N295D
87295pGADT7 Hsh155 H331DEncodes Y2H Gal4 activation domain for Hsh155 H331D
87296pGADT7 Hsh155 H331QEncodes Y2H Gal4 activation domain for Hsh155 H331Q
87297pGADT7 Hsh155 K335EEncodes Y2H Gal4 activation domain for Hsh155 K335E
87298pGADT7 Hsh155 K335NEncodes Y2H Gal4 activation domain for Hsh155 K335N
87299pGADT7 Hsh155 K335REncodes Y2H Gal4 activation domain for Hsh155 K335R
87300pGADT7 Hsh155 K335TEncodes Y2H Gal4 activation domain for Hsh155 K335T
87301pGADT7 Hsh155 P369EEncodes Y2H Gal4 activation domain for Hsh155 P369E
87302pGADT7 Hsh155 P369KEncodes Y2H Gal4 activation domain for Hsh155 P369K
87303pGADT7 Hsh155 D450GEncodes Y2H Gal4 activation domain for Hsh155 D450G
87547pGBKT7-BUD13Encodes Y2H Gal4 DNA binding domain for Bud13
87548pGBKT7-CLF1Encodes Y2H Gal4 DNA binding domain for Clf1
87557pGBKT7-CUS1Encodes Y2H Gal4 DNA binding domain for Cus1
87558pGBKT7-CUS2Encodes Y2H Gal4 DNA binding domain for Cus2
87573pGBKT7-HSH49Encodes Y2H Gal4 DNA binding domain for Hsh49
87588pGBKT7-MUD2Encodes Y2H Gal4 DNA binding domain for Mud2
87591pGBKT7-PRP2Encodes Y2H Gal4 DNA binding domain for Prp2
87592pGBKT7-PRP5Encodes Y2H Gal4 DNA binding domain for Prp5
87593pGBKT7-PRP9Encodes Y2H Gal4 DNA binding domain for Prp9
87594pGBKT7-PRP11Encodes Y2H Gal4 DNA binding domain for Prp11
87595pGBKT7-PRP28Encodes Y2H Gal4 DNA binding domain for Prp28
87596pGBKT7-PRP43Encodes Y2H Gal4 DNA binding domain for Prp43
87597pGBKT7-SLU7Encodes Y2H Gal4 DNA binding domain for Slu7
87598pGBKT7-YSF3Encodes Y2H Gal4 DNA binding domain for Ysf3
87599pRS413 Prp5 WTEncodes Prp5 WT for expression in yeast
87600pRS413 Prp5 AAAAEncodes Prp5 AAAA for expression in yeast
87601pRS413 Prp5 E235AEncodes Prp5 E235A for expression in yeast
87602pRS413 Prp5 N399DEncodes Prp5 N399D for expression in yeast
87603pRS413 Prp5 TAGEncodes Prp5 SAT motif to TAG for expression in yeast
87604pRS415 Prp2 Q548NEncodes Prp2 Q548N for expression in yeast

Antibodies from Article