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Lysine221 is the general base residue of the isochorismate synthase from Pseudomonas aeruginosa (PchA) in a reaction that is diffusion limited.

Meneely KM, Luo Q, Dhar P, Lamb AL
Arch Biochem Biophys. 2013 Oct 1;538(1):49-56. doi: 10.1016/j.abb.2013.07.026. Epub 2013 Aug 11. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
87102pET28b-pchA WTexpresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosa

Antibodies from Article