Genome-wide assessment of sequence-intrinsic enhancer responsiveness at single-base-pair resolution.

Arnold CD, Zabidi MA, Pagani M, Rath M, Schernhuber K, Kazmar T, Stark A
Nat Biotechnol. 2016 Dec 26. doi: 10.1038/nbt.3739. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
86380	pSTAP-seq_fly-ctrl	Vector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
86381	pSTAP-seq_fly-zfh1	Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
86382	pSTAP-seq_fly-ssp3	Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
86383	pSTAP-seq_fly-ham	Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
86384	pSTAP-seq_fly-sgl	Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
86385	pSTAP-seq_fly-ncm	Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
86386	pSTAP-seq_fly-tj	Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
86387	pSTAP-seq_fly-zfh1_spikeIn-1	Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
86388	pSTAP-seq_fly-zfh1_spikeIn-2	Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
86389	pSTAP-seq_fly-zfh1_spikeIn-3	Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
86390	pSTAP-seq_fly-zfh1_spikeIn-4	Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
86391	pGL3_zfh1_CP-candidate_luc+	Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity
86392	pGL3_ctrl_CP-candidate_luc+	Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates basal activity (no enhancer in vector) is reflected by luciferase activity