Rewiring human cellular input-output using modular extracellular sensors.
Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84500 | V1-MESA-45F-M-tTA | MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and tTA |
| 84501 | V1-MESA-45F-Tev | MESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev protease |
| 84502 | V2-MESA-35F-M-tTA | MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and tTA |
| 84503 | V2-MESA-35F-Tev | MESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev protease |
| 84504 | V1-MESA-45F-M-dCas9 | MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64 |
| 84505 | V1-MESA-45F-Tev-MS2-p65-HSF1 | MESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev protease, MS2-P65-HSF1 |
| 84506 | V2-MESA-35F-M-dCas9 | MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64 |
| 84513 | V2-MESA-35F-Tev-MS2-p65-HSF1 | MESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev protease, MS2-P65-HSF1 |