Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9.
Yumlu S, Bashir S, Stumm J, Kuhn R
Methods Mol Biol. 2019;1961:137-151. doi: 10.1007/978-1-4939-9170-9_10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86984 | pCAG-Trex2-bpA | Expression of mouse Trex2 |
| 86985 | U6-(BbsI)sgRNA_CAG-Venus-bpA | For cloning and expression of sgRNA together with Venus expression |
| 86986 | pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA | For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein |
| 86987 | pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP | For cloning and expression of sgRNA together with expression of Cas9 and TagRFP |
| 111144 | pCAG-Trex2-bpA-EF1BFP | Expression of mouse Trex2 and BFP reporter in mammalian cells |
| 111145 | pCAG-i53-bpA-EF1BFP | Expression of i53 and a BFP reporter in mammalian cells |