A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response.
Adamson B, Norman TM, Jost M, Cho MY, Nunez JK, Chen Y, Villalta JE, Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit A, Parnas O, Regev A, Weissman JS
Cell. 2016 Dec 15;167(7):1867-1882.e21. doi: 10.1016/j.cell.2016.11.048.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85967 | pBA439 | Perturb-seq vector backbone |
| 85968 | Perturb-seq GBC library | Perturb-seq GBC library |
| 85969 | pMH0001 | UCOE-SFFV-dCas9-BFP-KRAB |
| 85970 | pBA407 | UPRE-mCh-Ubc-Neo |
| 85971 | pBA409 | EF1a-sfGFP-Ubc-Neo |
| 85995 | pMJ114 | One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region |
| 85996 | pMJ179 | One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2 |
| 85997 | pMJ117 | One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3 |