Genetic and Transgenic Reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis.
Stern DL, Crocker J, Ding Y, Frankel N, Kappes G, Kim E, Kuzmickas R, Lemire A, Mast JD, Picard S
G3 (Bethesda). 2017 Apr 3;7(4):1339-1347. doi: 10.1534/g3.116.038885.
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PubMed
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
86860 | pBac{3XP3::EYFP,attP} | Expresses EYFP in eyes and contains an attP landing site for site-specific attB integration |
86861 | pBac{3XP3::EGFP, Pactin::Ptrsps} | Expresses EGFP in eyes and ubiquitous expression of P-transposase |
86862 | pBac{3XP3::DsRed, Pactin::Ptrsps} | Expresses DsRed in eyes and ubiquitous expression of P-transposase |
86863 | p{CFD4-EYFP-3xP3::DsRed} | Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site. |
86864 | p{CFD4-3xP3::DsRed} | Expresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site. |