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Genetic and Transgenic Reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis.

Stern DL, Crocker J, Ding Y, Frankel N, Kappes G, Kim E, Kuzmickas R, Lemire A, Mast JD, Picard S
G3 (Bethesda). 2017 Apr 3;7(4):1339-1347. doi: 10.1534/g3.116.038885. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
86860pBac{3XP3::EYFP,attP}Expresses EYFP in eyes and contains an attP landing site for site-specific attB integration
86861pBac{3XP3::EGFP, Pactin::Ptrsps}Expresses EGFP in eyes and ubiquitous expression of P-transposase
86862pBac{3XP3::DsRed, Pactin::Ptrsps}Expresses DsRed in eyes and ubiquitous expression of P-transposase
86863p{CFD4-EYFP-3xP3::DsRed}Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.
86864p{CFD4-3xP3::DsRed}Expresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.

Antibodies from Article