Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I
ACS Synth Biol. 2016 Dec 19.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84608 | pCRISPRyl_AXP | Introduces DSB at AXP locus in Y. lipolytica |
| 84609 | pCRISPRyl_XPR2 | Introduces DSB at XPR2 locus in Y. lipolytica |
| 84610 | pCRISPRyl_A08 | Introduces DSB at A08 locus in Y. lipolytica |
| 84611 | pCRISPRyl_D17 | Introduces DSB at D17 locus in Y. lipolytica |
| 84612 | pCRISPRyl_MFE1 | Introduces DSB at MFE1 locus in Y. lipolytica |
| 84613 | pHR_AXP_hrGFP | HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus |
| 84614 | pHR_XPR2_hrGFP | HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus |
| 84615 | pHR_A08_hrGFP | HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus |
| 84616 | pHR_D17_hrGFP | HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus |
| 84617 | pHR_MFE1_hrGFP | HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus |