Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells.
Ma Y, Zhang J, Yin W, Zhang Z, Song Y, Chang X
Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. Epub 2016 Oct 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83260 | MO91-dCas9-AID(P182X) | Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone. |