Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs.
Bertero A, Pawlowski M, Ortmann D, Snijders K, Yiangou L, Cardoso de Brito M, Brown S, Bernard WG, Cooper JD, Giacomelli E, Gambardella L, Hannan NR, Iyer D, Sampaziotis F, Serrano F, Zonneveld MC, Sinha S, Kotter M, Vallier L
Development. 2016 Dec 1;143(23):4405-4418.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86695 | pAAV-Puro_siKD | Tetracycline inducible expression of shRNA targeted to AAVS1 locus |
| 86696 | pAAV-Puro_siKO | Tetracycline inducible expression of guide RNA targeted to AAVS1 locus |
| 86697 | pAAV-Puro_siKO-2TO | Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus |
| 86698 | pAAV-Neo_CAG-Cas9 | Cas9 expression vector for targeting to the AAVS1 locus |