ESCRT III repairs nuclear envelope ruptures during cell migration to limit DNA damage and cell death.
Raab M, Gentili M, de Belly H, Thiam HR, Vargas P, Jimenez AJ, Lautenschlaeger F, Voituriez R, Lennon-Dumenil AM, Manel N, Piel M
Science. 2016 Apr 15;352(6283):359-62. doi: 10.1126/science.aad7611. Epub 2016 Mar 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86674 | pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A | Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events. |
| 86675 | pTRIP-CMV-GFP-FLAG-cGAS | Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events. |
| 86676 | pTRIP-CMV-tagRFP-FLAG-cGAS | Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events. |
| 86677 | pTRIP-SFFV-EGFP-NLS | Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events. |