An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis thaliana.
Hahn F, Mantegazza O, Greiner A, Hegemann P, Eisenhut M, Weber AP
Front Plant Sci. 2017 Jan 24;8:39. doi: 10.3389/fpls.2017.00039. eCollection 2017.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86555 | pFH6 | Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency |
| 86556 | pUB-Cas9 | For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017) |
| 86779 | pUB-Cas9-@GL1 | Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9 |