Gregor Bucher Lab: Bucher Lab plasmids
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86446 | pBac[3xP3 g Tc' v] | pBAc transposon with transformation marker for Tribolium transgenesis; Rescues vermilion white eyed mutant to black eyes; (transformation plasmid) |
| 86447 | pBac[6xP3-Tc-hsp-ECFP-SV40] | pBAc transposon with transformation marker for Tribolium transgenesis; drives ECFP (blue fluorescent) expression in the eyes (much stronger than usual 3XP3); (Transformation plasmid) |
| 86448 | pBac[6xP3-Tc-hsp-DsRedExpress-SV40] | pBAc transposon with transformation marker for Tribolium transgenesis; Drives dsRedExpress (red fluorescent) expression in the eyes (much stronger than usual 3XP3); (Transformation plasmid) |
| 86449 | pBac[3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR] | pBAc transposon with transformation marker for Tribolium transgenesis; Drives EGFP expression in the eyes; contains Gal4_ driven by Tribolium heatshock promoter; (Transformation plasmid) |
| 86450 | pSLfa[Tc'hsp5'3'UTR]fa | Heat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid) |
| 86451 | pSLfa[Hsp_p-GAL4Delta-SV40;attp]fa | Gal4 driver construct for misexpression in Tribolium with Tribolium basal promoter; has been used for enhancer trapping; (Shuttle plasmid) |
| 86452 | pSLfa[UAS-Tc'hsp_p-tGFP-SV40]fa | UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Shuttle plasmid); tGFP can be exchanged by other ORFs |
| 86453 | pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40] | UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker) |
| 86454 | pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP] | Nuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker) |
| 86455 | pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP] | Nuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker) |
| 86456 | pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP] | Lox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker) |