Analysis of membrane proteins localizing to the inner nuclear envelope in living cells.
Smoyer CJ, Katta SS, Gardner JM, Stoltz L, McCroskey S, Bradford WD, McClain M, Smith SE, Slaughter BD, Unruh JR, Jaspersen SL
J Cell Biol. 2016 Nov 21;215(4):575-590. Epub 2016 Nov 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86413 | pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1) | nuclear split GFP11 reporter with mCherry |
| 86414 | pSJ1679 (pRS315-NOP1pr-GFP11-PUS1) | nuclear split GFP11 reporter without mCherry |
| 86416 | pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM) | ONM/ER split GFP11 reporter with mCherry |
| 86417 | pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11) | luminal split GFP11 reporter with mCherry |
| 86418 | pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM) | ONM/ER split GFP1-10 |
| 86419 | pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX) | C-terminal PCR tagging module for yeast codon optimized split GFP1-10 |
| 86420 | pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10) | N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter |
| 86421 | pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10) | N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence |