NmeCas9 is an intrinsically high-fidelity genome-editing platform.
Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ
Genome Biol. 2018 Dec 5;19(1):214. doi: 10.1186/s13059-018-1591-1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86195 | pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer | U6 based expression of N meningitidis sgRNA |
| 115694 | pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS | All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter. |
| 120077 | pLenti_dNmeCas9 | dNmeCas9 with no sgRNA cassette lenti construct |
| 120078 | NmeCas9_3XNLS_pMCSG7 | Bacterial expression of wtNmeCas9 with 3X NLS |