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New families of single integration vectors and gene tagging plasmids for genetic manipulations in budding yeast.

Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
81089SIVuSingle Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.
81090SIVlSingle Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.
81091SIVhSingle Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.
81092SIVtSingle Integration Vector TRP - Cloning vector for single integration into the TRP1 locus of S.cerevisiae containing two MCS.
81093GTU-cGene Tagging vector URA/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
81094GTU-gGene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
81095GTU-yGene Tagging vector URA/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
81096GTU-rGene Tagging vector URA/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
81097GTU-irGene Tagging vector URA/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
81098GTL-cGene Tagging vector LEU/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
81099GTL-gGene Tagging vector LEU/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
81100GTL-yGene Tagging vector LEU/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
81102GTL-irGene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
81103GTH-cGene Tagging vector HIS/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
81104GTH-gGene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
81105GTH-yGene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
81106GTH-rGene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
81107GTH-irGene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
81108GTT-cGene Tagging vector TRP/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
81109GTT-gGene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
81110GTT-yGene Tagging vector TRP/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
81111GTT-rGene Tagging vector TRP/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
81112GTT-irGene Tagging vector TRP/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
81174MCVMCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 cloning of inserts
81195GTL-rGene Tagging vector LEU/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

Antibodies from Article