Dimerization and direct membrane interaction of Nup53 contribute to nuclear pore complex assembly.
Vollmer B, Schooley A, Sachdev R, Eisenhardt N, Schneider AM, Sieverding C, Madlung J, Gerken U, Macek B, Antonin W
EMBO J. 2012 Oct 17;31(20):4072-84. doi: 10.1038/emboj.2012.256. Epub 2012 Sep 7.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
85998 | pET28a SUMO Nup53xl 1-320 | bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53 |
85999 | pET28a MISTIC | bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag |
86000 | pSI HA Nup53xl | mammalian expression of HA tagged Xenopus laevis Nup53 |
86001 | pSI myc Nup53xl | mammalian expression of myc tagged Xenopus laevis Nup53 |