A computationally engineered RAS rheostat reveals RAS-ERK signaling dynamics.
Rose JC, Huang PS, Camp ND, Ye J, Leidal AM, Goreshnik I, Trevillian BM, Dickinson MS, Cunningham-Bryant D, Debnath J, Baker D, Wolf-Yadlin A, Maly DJ
Nat Chem Biol. 2016 Nov 21. doi: 10.1038/nchembio.2244.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 40775 | Braf | |
| 86502 | CIAR_pcDNA5/FRT/TO | Expresses CIAR in mammalian cells. Used to generate Flp-In stables. |
| 86503 | GFP-P2A-CIAR_pLenti_CMV_Puro | Lenti plasmid for generating CIAR expressing stable cell lines |
| 86504 | xCIAR_pcDNA5/FRT/TO | Expresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables. |
| 86505 | CRAF_S259A_pcDNA3.1 | Expresses CRAF_S259A in mammalian cells |
| 86506 | CRAF_S29A_pcDNA3.1 | Expresses CRAF_S29A in mammalian cells [Edit] |