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Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana.

Hopes A, Nekrasov V, Kamoun S, Mock T
Plant Methods. 2016 Nov 24;12:49. doi: 10.1186/s13007-016-0148-0. eCollection 2016. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
85981pCR8/GW:U6Golden Gate Level 0 cassette encoding the U6 promoter
85982pAGM4723:TpCC_UreaseGolden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs
85984pICH47732:FCP:NATGolden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter
85986pICH47742:FCP:Cas9YFPGolden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

Antibodies from Article