A bacterial genetic selection system for ubiquitylation cascade discovery.
Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T, Katina C, Pilzer I, Ben-Aroya S, Prag G
Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 2016 Oct 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83073 | pGST-DHFR Mm | Expresses the full length DHFR Mm in E.coli |
| 83232 | pGEN25-Ub Hs | pHis-par2-Ub Hs |
| 83233 | pCOG75-Sem1 Sc | pCDF-His6-MBP-Sem1 Sc |
| 83234 | pGEN6-Ubc5 Sc | pHis-par2-UbHs/Ubc5Sc/ Uba1Ta |
| 83235 | pSc-His6-Sem1Sc | pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast |
| 83236 | pCOG40-HRS (257-276) Hs | pGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coli |