The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex.
Tie HC, Madugula V, Lu L
Biochem Biophys Res Commun. 2016 Sep 30;478(4):1694-9. doi: 10.1016/j.bbrc.2016.09.005. Epub 2016 Sep 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87328 | pSUPER-retro-puro-shNup88-XbaI | To express shRNA against human Nup88 |
| 87329 | pSUPER-retro-puro-shNup88-HindIII | To express shRNA against human Nup88 |
| 87330 | pSUPER-retro-puro-shNup93-XhoI | To express shRNA against human Nup93 |
| 87331 | pSUPER-retro-puro-shNup93-SpeI | Expression of shRNA targeting at Nup93 |
| 87332 | pSUPER-retro-puro-shNup35-EcoRI | To express shRNA against human Nup35 |
| 87333 | pSUPER-retro-puro-shNup133-2 | To express shRNA against human Nup133 |
| 87334 | pSUPER-retro-puro-shNup133-1 | To express shRNA against human Nup133 |
| 87335 | pEGFP-C1X-Nup93-mut | To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA |
| 87336 | pEGFP-C1X-Nup93 | To express human Nup93 in mammalian cells |
| 87337 | pEGFP-C1X-Nup88-mut | To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA |
| 87338 | pEGFP-C1X-Nup88 | To express human Nup88 in mammalian cells |
| 87339 | pEGFP-C1-KSENB-Nup133-mut | To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA |
| 87340 | pEGFP-C1-KSENB-Nup133 | To express human Nup133 in mammalian cells |
| 87341 | pEGFP-C1-Nup35-mut | To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA |
| 87342 | pEGFP-C1-Nup35 | To express human Nup35 in mammalian cells |
| 87343 | pEGFP-C1-6xHis | To express EGFP-6xHis in mammalian cell |