CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and Functional Analysis of Oncogenic Driver Mutations.

Guernet A, Mungamuri SK, Cartier D, Sachidanandam R, Jayaprakash A, Adriouch S, Vezain M, Charbonnier F, Rohkin G, Coutant S, Yao S, Ainani H, Alexandre D, Tournier I, Boyer O, Aaronson SA, Anouar Y, Grumolato L
Mol Cell. 2016 Aug 4;63(3):526-38. doi: 10.1016/j.molcel.2016.06.017. Epub 2016 Jul 21. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
85571	sgAAVS1(B)	Expresses sgRNA targeting human AAVS1 locus
85570	sgAAVS1(A)	Expresses sgRNA targeting human AAVS1 locus
85569	sgALK(Chr. Inv.)	Expresses sgRNA targeting human ALK between exon 20 and 21
85568	sgEML4	Expresses sgRNA targeting human EML4 between exon 13 and 14
85567	sgKRAS(B)	Expresses sgRNA targeting human KRAS around codon G12
85566	sgKRAS(A)	Expresses sgRNA targeting human KRAS around codon G12
85565	sgEGFR(B)	Expresses sgRNA targeting human EGFR around codon T790
85564	sgEGFR(A)	Expresses sgRNA targeting human EGFR around codon T790
85563	sgALK(B)	Expresses sgRNA targeting human ALK around codon F1174
85562	sgALK(A)	Expresses sgRNA targeting human ALK around codon F1174