Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain.
Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 10.1073/pnas.1522985113. Epub 2016 May 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78269 | pSEVA331Bb | pSEVA331 with Biobrick multiple cloning site |
| 78271 | J23100-mRFP1-331Bb | mRFP1 behind constitutive promoter J23100 in pSEVA331Bb backbone |
| 78272 | J23101-mRFP1-331Bb | mRFP1 behind constitutive promoter J23101 in pSEVA331Bb backbone |
| 78273 | J23103-mRFP1-331Bb | mRFP1 behind constitutive promoter J23103 in pSEVA331Bb backbone |
| 78274 | J23104-mRFP1-331Bb | mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone |
| 78275 | J23105-mRFP1-331Bb | mRFP1 behind constitutive promoter J23105 in pSEVA331Bb backbone |
| 78277 | J23110-mRFP1-331Bb | mRFP1 behind constitutive promoter J23110 in pSEVA331Bb backbone |
| 78278 | J23112-mRFP1-331Bb | mRFP1 behind constitutive promoter J23112 in pSEVA331Bb backbone |
| 78279 | J23114-mRFP1-331Bb | mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone |
| 78280 | J23117-mRFP1-331Bb | mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone |
| 78281 | pLux01 | mRFP1 expressed behind an AHL-inducible construct |
| 78282 | pLux02 | mRFP1 expressed behind an AHL-inducible construct |
| 78283 | pTet01 | mRFP1 expressed behind an ATc-inducible construct |
| 78284 | pTet02 | mRFP1 expressed behind an ATc-inducible construct |
| 78285 | J-sRNA-331Bb | An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNA |