Generating permissive site-specific unnatural aminoacyl-tRNA synthetases.

Miyake-Stoner SJ, Refakis CA, Hammill JT, Lusic H, Hazen JL, Deiters A, Mehl RA
Biochemistry. 2010 Mar 2;49(8):1667-77. doi: 10.1021/bi901947r. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
85484	pDule-tfmF A65V S158A	Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
85483	pBad-sfGFP 150TAG	Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150
85482	pBad-sfGFP	Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag