Systematic mapping of functional enhancer-promoter connections with CRISPR interference.
Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, Kane M, Cleary B, Lander ES, Engreitz JM
Science. 2016 Sep 29. pii: aag2445.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85449 | TRE-KRAB-dCas9-IRES-BFP | Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP |
| 85556 | TRE-KRAB-dCas9-IRES-GFP | 2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP |
| 85681 | sgOpti | Lentiviral vector to express sgRNAs. The cloning site is BsmBI. |
| 86461 | pGL4.23-MYC | A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site. |
| 120341 | pGL4.23-MYC-e3 | pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter. |
| 120342 | pGL4.23-MYC-e4 | pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter. |
| 120343 | pGL4.23-MYC-e5 | pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter. |