Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins.
Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
84354 | CyRFP1-C1 | Expresses CyRFP1 fluorescent protein in an empty backbone |
84355 | mCyRFP1-C1 | Expresses mCyRFP1 fluorescent protein in an empty backbone |
84356 | pCAG-CyRFP1 | Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter |
84357 | AAV-FLEX-CAG-CyRFP1 | Expresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent) |
84358 | mCyRFP1-RhoA | fusion protein of RhoA, FRET donor |
84359 | mMaroon-RTK-mMaroon | FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon |
84360 | mCyRFP1-Cdc42 | fusion protein of Cdc42, FRET donor |
84361 | mMaroon-PAK-mMaroon | FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon |
84486 | mCyRFP1-mMaroon | Direct FRET pair Fusion mCyRFP1-mMaroon |
84545 | pNCS-mCyRFP1 | Bacterial expression of mCyRFP1 |
84546 | pNCS-CyRFP1 | Bacterial expression of CyRFP1 |