Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85405 | pGH020_sgRNA_G418-GFP | hu6 driven sgRNA vector with G418 and GFP selectable markers |
| 85406 | pGH335_MS2-AID*Δ-Hygro | lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker |
| 85407 | pGH314_wtGFP S65T, Q80H | lentiviral expression vector for wtGFP S65T-Q80H |
| 85408 | pGH311_wtGFP S65T | lentiviral expression vector for wtGFP S65T |
| 85409 | pGH312_wtGFP Q80H | lentiviral expression vector for wtGFP Q80H |
| 85410 | pGH220_wtGFP | lentiviral expression vector for wtGFP |
| 85411 | pGH045_EGFP | lentiviral expression vector for GFP |
| 85412 | pGH044_mCherry | lentiviral expression vector for mCherry |
| 85413 | pGH224_sgRNA_2xMS2_Puro | hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker |
| 85414 | pGH183_MS2-AIDΔDead-Hygro | lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker |
| 85415 | pGH156_MS2-AID-Hygro | lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker |
| 85416 | pGH153_MS2-AIDΔ-Hygro | lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker |
| 85417 | pGH125_dCas9-Blast | lentiviral expression vector for dCas9 with Blasticidin selectable marker |