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Visualization of retrovirus uptake and delivery into acidic endosomes.

Miyauchi K, Marin M, Melikyan GB
Biochem J. 2011 Mar 15;434(3):559-69. doi: 10.1042/BJ20101588. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
85389EcpH-TMExpresses ecliptic pHluorin–ICAM-1 (intercellular adhesion molecule 1) in mammalian cells. Incorporates into viral membrane to label pseudoviruses
85390HIV-1 Gag-mCherryVisualization marker for viral core to measure cellular uptake

Antibodies from Article