Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.
Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84239 | pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9 | Expresses ABA-inducible VPR-Sp dCas9 |
| 84240 | pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9 | Expresses GA-inducible VPR-Sp dCas9 |
| 84241 | pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9 | Expresses ABA-inducible KRAB-Sp dCas9 |
| 84242 | pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9 | Expresses GA-inducible KRAB-Sp dCas9 |
| 84243 | pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9 | Expresses ABA-inducible VPR-Sa dCas9 |
| 84244 | pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9 | Expresses GA-inducible VPR-Sa dCas9 |
| 84245 | pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9 | Expresses direct fusion KRAB-Sp dCas9 |
| 84246 | pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9 | Expresses direct fusion VPR-Sa dCas9 |
| 84247 | pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9 | Expresses direct fusion VPR-Sp dCas9 |
| 84248 | pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry | Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker |
| 84249 | pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry | Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker |
| 84250 | pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry | Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker |
| 84251 | pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP | Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker |
| 84252 | pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP | Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker |
| 84253 | pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP | Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker |
| 84254 | pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP | Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker |
| 84255 | pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFP | Expresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent marker |
| 84256 | pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP | Expresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker |
| 84257 | pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI | Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation |
| 84258 | pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry | Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate |
| 84260 | pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry | Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation |
| 84261 | pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9 | Expresses AND gate ABA/GA-inducible VPR-Sp dCas9 |
| 106895 | pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI | Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation |