Skip to main content
Addgene

Engineered calcium-precipitable restriction enzyme.

Hendrix J, Read T, Lalonde JF, Jensen PK, Heymann W, Lovelace E, Zimmermann SA, Brasino M, Rokicki J, Dowell RD
ACS Synth Biol. 2014 Dec 19;3(12):969-71. doi: 10.1021/sb500042m. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
85165pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCPProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
85166pSB1A3 EcoRI Methylase-AmilCPFor methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

Antibodies from Article