Dynamic localization of the cyanobacterial circadian clock proteins.
Cohen SE, Erb ML, Selimkhanov J, Dong G, Hasty J, Pogliano J, Golden SS
Curr Biol. 2014 Aug 18;24(16):1836-44. doi: 10.1016/j.cub.2014.07.036. Epub 2014 Aug 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85105 | pAM5084 | Ptrc-ECFP-KaiC fusion cloned into Km resistance gene of pACYC177. To look for KaiC localization in E. coli. |
| 85106 | pAM5080 | Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres |
| 85107 | pAM5081 | Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels. |
| 85121 | pAM5082 | PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp |
| 85122 | pAM5085 | PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period. |
| 85123 | pAM5087 | Ptrc-YFP subcloned from pAM5080 into NSII-Km vector. |
| 85124 | pAM4645 | NSII vector expressing PkaiB-KaiBC. Nt resistant. Complements rhthyms in AMC705 |
| 85125 | pAM5089 | KaiB expressed from native promoter from NSII-Nt. Complements rhythms of kaiB null strain. |