Human METTL20 is a mitochondrial lysine methyltransferase that targets the beta subunit of electron transfer flavoprotein (ETFbeta) and modulates its activity.
Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115. Epub 2014 Nov 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85109 | pF708-pET28a-Hs-delta38-METTL20-NHis wt | expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag |
| 85110 | pF709-pET28a-Hs-FL-ETFbeta-NHis wt | expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag |
| 85111 | pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt | expression of recombinant human mature (delta19) ETFalpha wt without tag |
| 85112 | pF711-pET28a-Hs-delta25-MCAD-NHis wt | expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag |
| 85113 | pF712-pET28a-Hs-delta44-GCDH-NHis wt | expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag |
| 85114 | pF713-pEGFP-N1-Hs-FL-METTL20 wt | transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion |
| 85115 | pF714-pET28a-Hs-FL-METTL20-NHis wt | expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag |