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Dorus Gadella Lab: Gadella lab plasmids

Unpublished

Plasmids from Article

ID Plasmid Purpose
98832CytERM-mKOkappaIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
112936LSS-SGFP2-aTubVisualization of microtubules
112938LifeAct-LSS-SGFP2-N1Visualization of F-actin
112939pRSETb-LSS-SGFP2Bacterial expression vector for LSS-SGFP2
112940CytERM-LSS-SGFP2In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
112941pRSETb-LssmsfGFPBacterial expression vector for LSS-msfGFP
112942pRSETb-LSS-mEGFPBacterial expression vector for LSS-EGFP
112943mCherry-LSS-SGFP2Produces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRET
112944mCherry-T2A-LSS-SGFP2Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
112945mScarlet-I-LSS-SGFP2Produces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRET
112946mScarlet-I-T2A-LSS-SGFP2Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
112947RhoA sensor (LSS-SGFP2-Scarlet-I)RhoA FRET biosensor
112948RhoA sensor (LSS-SGFP2-mCherry)RhoA FRET biosensor
112949pmCherry-Ggamma2mCherry-Ggamma2 fusion
112950mScarlet-I-Ggamma2mScarlet-I-Ggamma2 fusion
112951Gaq-LSS-SGFP2Galphaq tagged with LSS-SGFP2
112952RhoA sensor (mScarlet-I_10_LSS-SGFP2_10)RhoA FRET biosensor
112953pTfR-mTurquoise2In vivo visualization of Transferrin receptors (can be used for colocalization studies)
112954pTfR-mScarlet-IIn vivo visualization of Transferrin receptors (can be used for colocalization studies)
112958CytERM_3xmTurquoise2 In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
176093miRFP670 - H2ANucelear marker
176105LifeAct-HaloTagF-actin marker
176111SspB-mCherry-DHPH1-TRIOHeterodimerization with iLID, Rac1 and RhoG activation
176115SspB-HaloTag-DHPH1-TRIOHeterodimerization with iLID, Rac1 and RhoG activation
176119SspB-iRFP-DHPH1-TRIOHeterodimerization with iLID, Rac1 and RhoG activation
176126pLV-SspB-HaloTag-TRIO-DHPH1Heterodimerization with iLID, Rac1 and RhoG activation
176133pEntr1a-SspB-HaloTag-TRIO-DHPH1Heterodimerization with iLID, Rac1 and RhoG activation

Antibodies from Article